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cat vs. pH profile, only one ionization is observed for either Zn- or Cd-NagA. This ionization will have to stand for the protonation of the steel-bound hydroxide that's used for substrate hydrolysis or, alternatively, the overall foundation that may be utilized to deprotonate the steel-certain water molecule previous to substrate hydrolysis. The p
utilizes just one metal certain solely on the β-web-site. One of the most Serious instance within the amidohydrolase superfamily is uronate isomerase from E. coli
m values have been identified from suits of the info to the straight line. Saturation curves demonstrating substrate inhibition ended up in shape to equation two have been K
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